HuT 78細(xì)胞

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

年限： 53 years *****

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

ATCC Number： TIB-161?

相關(guān)**： 其他**

細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Designations: HuT 78

HuT 78細(xì)胞Depositors: AF Gazdar

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: Sezary Syndrome

Cell Type: cutaneous T lymphocyte;

HuT 78細(xì)胞Cellular Products: interleukin 2 [1140]

tumor necrosis factor alpha (TNF alpha) [23420]

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Applications: transfection host

Receptors: interleukin 2 (IL-2), expressed [1140]

Tumorigenic: Yes

Antigen Expression: CD4; Homo sapiens [22610]

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 8,12

D16S539: 11,12

D5S818: 11,12

D7S820: 8,11

THO1: 8,9

TPOX: 8,9

vWA: 14,15

Age: HuT 78細(xì)胞53 years *****

Gender: male

Ethnicity: Caucasian

Comments: H9 (ATCC HTB-176) is a clonal derivative of HuT 78 [Pubmed: 2567177].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp4 and 8 X 10 exp5 viable cells/ml. maintain cell density at less than 1 X 10 exp6 cells/ml.

Medium Renewal: Two to three times weekly

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: about 65 hours

Related Products: HuT 78細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

derivative:ATCC HTB-176

References: 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

22484: Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177

22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

23228: Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674

23420: O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285

32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

32796: Bloom TJ, Beavo JA. HuT 78細(xì)胞Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082