RD細(xì)胞

數(shù)量： 大量

組織來源： muscle

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 其他

ATCC Number： CCL-136?

相關(guān)**： 橫紋肌肉瘤

是否是腫瘤細(xì)胞： 1

物種來源： 人

運(yùn)輸方式： 凍存運(yùn)輸

年限： 7 years

Designations: RD

RD細(xì)胞Depositors: RM McAllister

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: spindle cells and large multinucleated cells

Source: Tissue: muscle

Disease: rhabdomyosarcoma

Cellular Products: myoglobin

myosin ATPase

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RD細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: detection of viruses

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 13

D16S539: 10,11

D5S818: 11

D7S820: 8,12

THO1: 9.3

TPOX: 9

vWA: 18

Isoenzymes: G6PD, B

Age: 7 years

Gender: female

Ethnicity: Caucasian

Propagation: RD細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

22219: McAllister RM, et al. RD細(xì)胞Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949

22238: Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733

22929: Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385

23221: Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033

26001: . . J. Virol. 1: 326, 1967.