MNNG/HOS (Cl #5)細胞

年限： 13 years

是否是腫瘤細胞： 1

物種來源： 人

數量： 大量

運輸方式： 凍存運輸

器官來源： 骨

ATCC Number： CRL-1547?

相關**： 骨肉瘤

生長狀態： 貼壁生長

Designations: MNNG/HOS Cl #5 [R-1059-D]

Depositors: JS Rhim

MNNG/HOS (Cl #5)細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: bone

Disease: osteosarcoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

MNNG/HOS (Cl #5)細胞D13S317: 12

D16S539: 10,13

D5S818: 13

D7S820: 11,12

THO1: 6

TPOX: 8,11

vWA: 18

Isoenzymes: G6PD, B

Age: 13 years

Gender: female

Ethnicity: Caucasian

Comments: This line was derived from HOS (ATCC CRL-1543) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.

Propagation: MNNG/HOS (Cl #5)細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.

References: 22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046

26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298

32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. MNNG/HOS (Cl #5)細胞Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224