SNU-398細(xì)胞

年限： 42 years

器官來源： 肝

生長狀態(tài)： 混合型生長

是否是腫瘤細(xì)胞： 1

物種來源： 人

數(shù)量： 大量

細(xì)胞形態(tài)： 上皮樣

ATCC Number： CRL-2233?

相關(guān)**： 肝癌

運(yùn)輸方式： 凍存運(yùn)輸

SNU-398細(xì)胞Designations: SNU-398

Depositors: J Park

Biosafety Level: 2 [CELLS CONTAIN HEPATITIS B VIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed adherent and floating

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: liver

Disease: hepatocellular carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SNU-398細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: This line is available under the following restrictions: 1.) The cell line was deposited for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. 2.) Any proposed commercial use of these cells or products produced by them must first be negotiated with Jae-GaHB-Park, Director, Korean Cell Line Bank, 28 Yongon-dong, Chongno-gu, Seoul, 110-744 Korea. Telephone (02) 760-3380, Fax (02) 742-4727. 3.) In all papers reporting any use of these cells or derived products, a direct reference will be made to the original publication (Int. J. Cancer 62:276-282, 1995).

Antigen Expression: Blood Type O; Rh +

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 13

D13S317: 11

D16S539: 10,14

D5S818: 12

D7S820: 10,11

THO1: 7,9

TPOX: 11

vWA: 17,18

Cytogenetic Analysis: SNU-398細(xì)胞aneuploid; modal number = 62

Age: 42 years

Gender: male

Ethnicity: Asian

Comments: SNU-398 was derived in 1990 by J.-G. Park and associates from an anaplastic hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum.

After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.

Grossly, the original tumor was single nodular with perinodular extensions.

Histologically, it was trabecular type.

The cultured cells are multinucleated, and maintain the production of intracytoplasmic hyaline globules as seen in the original tumor.

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.

HBV genomic RNA was not expressed.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10%

Subculturing: SNU-398細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.

Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Doubling Time: 39 hrs

References: 22872: Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080