NCI-H520細胞
運輸方式： 凍存運輸
細胞形態： 上皮樣
生長狀態： 貼壁生長
是否是腫瘤細胞： 1
物種來源： 人
器官來源： 肺
數量： 大量
ATCC Number： HTB-182?
相關**： 鱗狀細胞癌
Designations: NCI-H520 [H520]
Depositors: AF Gazdar, JD Minna
NCI-H520細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: squamous cell carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 10,11
D16S539: 8,13
D5S818: 12,13
NCI-H520細胞D7S820: 8,12
THO1: 10
TPOX: 8
vWA: 18,19
Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells. Among the common markers were 1q+, t(1q8q), 2q+, der(16)t(3;16)(q21;q22), der(19)t(13;19)(q21;q13), and der(5)t(5;5)(p15pq13). Generally, there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent, and the X chromosome was single.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 1
PGM3, 1
Gender: male
Comments: The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung.
The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
The line can be cloned in soft agar (with or without serum).
Propagation: NCI-H520細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: 61 hrs in medium with serum; 25 to 32 hrs in serum-free medium
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
22434: Brower M, et al. NCI-H520細胞Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644
