SW756細(xì)胞
ATCC Number： CRL-10302?
相關(guān)**： 鱗狀細(xì)胞癌
數(shù)量： 大量
器官來源： 宮頸
是否是腫瘤細(xì)胞： 1
物種來源： 人
細(xì)胞形態(tài)： 上皮樣
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
年限： 46 years
Designations: SW756
Depositors: Univ. Texas System, Board of Regents
SW756細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cervix
Disease: squamous cell carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Temple Texas, United States
Isolation date: 1974
Virus Susceptibility: Influenza A virus
Tumorigenic: Yes
Antigen Expression: SW756細(xì)胞HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 17
Cytogenetic Analysis: modal number = 76; range = 60 to 96
Isoenzymes: G6PD, B
GLO-I, 1-2
Age: 46 years
Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
SW756細(xì)胞Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22075: Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995
22667: Freedman RS, et al. SW756細(xì)胞Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487
