C2BBe1細胞

是否是腫瘤細胞： 1

物種來源： 人

數量： 大量

生長狀態： 貼壁生長

ATCC Number： CRL-2102?

相關**： 大腸癌

年限： 72 years

運輸方式： 凍存運輸

細胞形態： 上皮樣

器官來源： 結腸

Designations: C2BBe1 [clone of Caco-2 (ATCC HTB-37)]

Depositors: MD Peterson, M Mooseker

Biosafety Level: 1

Shipped: frozen

C2BBe1細胞Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Disease: colorectal adenocarcinoma

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Applications: C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.

Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.

The cells express substantial levels of BB myosin I similar to that of the human enterocyte.

Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.

Receptors: epidermal growth factor (EGF)

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11, 13, 14

D16S539: 12, 13

D5S818: 12, 13

C2BBe1細胞D7S820: 11, 12

THO1: 6

TPOX: 9, 11

vWA: 16, 18

Age: 72 years

Gender: male

Ethnicity: Caucasian

Comments: The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line (see ATCC HTB-37) by limiting dilution.

The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.

C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.

Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.

The cells express substantial levels of BB myosin I similar to that of the human enterocyte.

Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human transferrin; fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: C2BBe1細胞Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subculture at 85% of confluence (about every 5 to 7 days).

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC HTB-37

References: 23012: Basson MD, et al. C2BBe1細胞Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287

23149: Peterson MD, Mooseker MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600, 1992. PubMed: 1506435