SW1417細(xì)胞

器官來(lái)源： 結(jié)腸

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： Dukes' type C, grade III

ATCC Number： CCL-238?

數(shù)量： 大量

相關(guān)**： 大腸癌

Designations: SW1417 [SW-1417]

Depositors: A Leibovitz

SW1417細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Tumor Stage: Dukes' type C, grade III

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) 362 ng/10 exp6 cells/10 days; keratin

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Tumorigenic: No

Oncogene: myc +; ras +; fos +; sis +; p53 -; myb -; abl -; ros -; src -

Antigen Expression: blood type B; Rh+

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 1

Age: 53 years

Gender: female

Ethnicity: SW1417細(xì)胞Caucasian

Comments: CSAp negative (CSAp-).

Colon antigen 3, negative.

The cells are positive for keratin by immunoperoxidase staining.

Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.

The line is positive for expression of c-myc, H-ras, sis and fos oncogenes.

N-myc, K-ras, N-ras and myb oncogene expression were not detected.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 1 to 2 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. SW1417細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

23074: Dahiya R, et al. SW1417細(xì)胞ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345