NCI-H1688細胞

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： CCL-257?

相關**： 小細胞肺癌

生長狀態： 貼壁生長

數量： 大量

年限： 50 years

細胞形態： 上皮樣

器官來源： 肺

運輸方式： 凍存運輸

Designations: NCI-H1688 [H1688]

NCI-H1688細胞Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: carcinoma; classic small cell lung cancer

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

NCI-H1688細胞D13S317: 8

D16S539: 13

D5S818: 10

D7S820: 12

THO1: 6,8

TPOX: 11

vWA: 17

Age: 50 years

Gender: male

Ethnicity: Caucasian

Comments: This line was derived from a liver metastasis taken from a patient prior to therapy.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; NCI-H1688細胞carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature.

Remove the trypsin and incubate the flask at 37C for 5 to 10 minutes or until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:NCI-H1688細胞ATCC 30-2020