NTERA-2 cl.D1細胞

ATCC Number： CRL-1973?

相關**： 其他**

數量： 大量

生長狀態： 貼壁生長

細胞形態： 其他

器官來源： 睪丸

是否是腫瘤細胞： 1

物種來源： 人

年限： 22 years

運輸方式： 凍存運輸

Designations: NTERA-2 cl.D1 [NT2/D1]

Depositors: PW Andrews

NTERA-2 cl.D1細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial-like,differentiation changes phenotype

Source: Organ: testis

Disease: malignant pluripotent embryonal carcinoma

Derived from metastatic site: lung

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Applications: transfection host

Virus Resistance: UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13

D16S539: 11,12,13

D5S818: 9,12

NTERA-2 cl.D1細胞D7S820: 10,12

THO1: 9.3

TPOX: 8

vWA: 18,19

Cytogenetic Analysis: This is a hypotriploid human cell line with the modal chromosome number of 63 in 48% of cells examined. However, cells with 62 chromosome counts also occurred at a rather high frequency (24%). The rate of polyploidy was 1.6%., About 12 marker chromosomes are constantly found in most cells. They include: der(9)t(1;9)(q25;q34.3); del(1)(q25); der(13)t(11;13)(q13;q34); t(Xq1q); and eight others., At least two markers are found only in some cells. The normal Y chromosome was found in all cells. Only single copies of normal chromosomes 1, 10, 11 and 13 were present. Others were mostly in two or three copies per cell.

Age: 22 years

Gender: male

Ethnicity: Caucasian

Comments: The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line.

The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line (see ATCC HTB-106).

This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide ((HMBA).

The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters.

The cells exhibit high expression of N-myc oncogene activity.

To induce differentiation, the cells should be trypsinized and seeded at a density 1 X 10 exp6 cells per 75 sq. cm. in medium containing 0.01 mM trans-retinoic acid.

Stock solutions of trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen (preferably under a nitrogen atmosphere).

Propagation: NTERA-2 cl.D1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Subcultures are prepared by scraping.

Cells from confluent cultures (approximately 20 million cells per 75 sq. cm.) are dislodged from the flask surface, aspirated and dispensed into new flasks.

Cultures should be maintained at high density.

Seed new flasks at a density of at least 5 X 10 exp6 viable cells per 75 sq. cm. flask.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22321: Andrews PW. Human teratocarcinomas. Biochim. Biophys. Acta 948: 17-36, 1988. PubMed: 3293662

22336: Mavilio F, et al. Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37: 73-79, 1988. PubMed: 2898410

22407: Fenderson BA, et al. Glycolipid core structure switching from globo- to lacto- and ganglio- series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells. Dev. Biol. 122: 21-34, 1987. PubMed: 3297853

22741: Andrews PW, et al. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab. Invest. 50: 147-162, 1984. PubMed: 6694356

22746: Andrews PW, et al. Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation 43: 131-138, 1990. PubMed: 2373286

22947: Gonczol E, et al. Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224: 159-161, 1984. PubMed: 6322309

22999: Andrews PW. NTERA-2 cl.D1細胞Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev. Biol. 103: 285-293, 1984. PubMed: 6144603

23235: Hirka G, et al. Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by human cytomegalovirus coinfection. J. Virol. 65: 2732-2735, 1991. PubMed: 1850047

29000: Dewji NN, Singer SJ. Cell surface expression of the Alzheimer disease-related presenilin proteins. Proc. Natl. Acad. Sci. USA 94: 9926-9931, 1997. PubMed: 9275228

49353: Baldassarre G, et al. Transfection with a CRIPTO anti-sense plasmid suppresses endogenous CRIPTO expression and inhibits transformation in a human embryonal carcinoma cell line. Int. J. Cancer 66: 538-543, 1996. PubMed: 8635871