H9/HTLV-IIIB細胞

生長狀態： 懸浮生長

數量： 大量

ATCC Number： CRL-8543?

細胞類型： T**細胞

是否是腫瘤細胞： 1

物種來源： 人

運輸方式： 凍存運輸

細胞形態： **樣

Designations: H9/HTLV-IIIB

H9/HTLV-IIIB細胞Depositors: The United States of America

Biosafety Level: 2 [Cells Contain RETROVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Cell Type: T lymphocyte;

Cellular Products: Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV)

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Comments: H9/HTLV-IIIB細胞The cells produce HTLV III.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

H9/HTLV-IIIB細胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3536: Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985

22938: Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298

32754: Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. H9/HTLV-IIIB細胞PubMed: 8551569