U-118 MG細胞
數量： 大量
器官來源： 大腦
運輸方式： 凍存運輸
生長狀態： 貼壁生長
年限： classified as grade IV as of 2007
細胞形態： 混合型
ATCC Number： HTB-15?
相關**： 其他**
是否是腫瘤細胞： 1
物種來源： 人
Designations: U-118 MG
U-118 MG細胞Depositors: J Ponten
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: mixed
Source: Organ: brain
Tumor Stage: classified as grade IV as of 2007
Disease: glioblastoma; astrocytoma
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Tumorigenic: Yes
Antigen Expression: Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47
DNA Profile (STR): U-118 MG細胞Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18
Isoenzymes: AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 2
Age: 50 years
Gender: male
Ethnicity: Caucasian
Comments: NOTE: U-118 MG細胞The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.
U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-16) by J. Ponten and associates from 1966 to 1969.
Mycoplasma contamination was eliminated in 1987 by treatment with BM-Cycline over a six week culture period.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapot temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22159: Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744
22536: Fogh J, et al. U-118 MG細胞Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
23128: Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
23260: Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013
32276: Cairns P, et al. U-118 MG細胞Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
