COLO 320DM細(xì)胞
數(shù)量： 大量
年限： Dukes' type C
細(xì)胞形態(tài)： 其他
生長狀態(tài)： 混合型生長
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CCL-220?
相關(guān)**： 大腸癌
器官來源： 結(jié)腸
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: COLO 320DM
Depositors: GE Moore
COLO 320DM細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens
Morphology: rounded and refractile
Source: Organ: colon
Tumor Stage: Dukes' type C
Disease: colorectal adenocarcinoma
Cellular Products: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
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Tumorigenic: Yes
DNA Profile (STR): COLO 320DM細(xì)胞Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 9
TPOX: 8,9
vWA: 15,18
Cytogenetic Analysis: modal number = 24; range = 49 to 61.
The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
COLO 320DM細(xì)胞PGM1, 1
PGM3, 2
Age: 55 years
Gender: female
Ethnicity: Caucasian
Comments: The line is negative for CEA, CSAp and colon antigen 3.
The cells are weakly positive for keratins and vimentin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.
Add 2.0 to 3.0 of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: COLO 320DM細(xì)胞Liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117
22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
