U-2 OS細(xì)胞
ATCC Number： HTB-96?
相關(guān)**： 骨肉瘤
數(shù)量： 大量
是否是腫瘤細(xì)胞： 1
物種來源： 人
器官來源： 骨
生長狀態(tài)： 貼壁生長
運(yùn)輸方式： 凍存運(yùn)輸
年限： 15 years
細(xì)胞形態(tài)： 上皮樣
Designations: U-2 OS
Depositors: Hellstrom
U-2 OS細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: bone
Disease: osteosarcoma
Cellular Products: osteosacoma derived growth factor (ODGF)
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Applications: transfection host
Receptors: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II)
Antigen Expression: Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
DNA Profile (STR): U-2 OS細(xì)胞Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18
Cytogenetic Analysis: Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others. [22509]
U-2 OS細(xì)胞Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 2
PGM3, 1
Age: 15 years
Gender: female
Ethnicity: Caucasian
Comments: J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses.
Mycoplasma contamination was detected and eliminated in 1972.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: U-2 OS細(xì)胞2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 22237: Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080
22509: Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590
23011: Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159: 531-541, 1994. PubMed: 8188767
32288: Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
32308: Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487
