ChaGo-K-1細胞
年限： 45 years
器官來源： 肺
組織來源： bronchus
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： HTB-168?
運輸方式： 凍存運輸
相關**： 其他**
生長狀態： 貼壁生長
細胞形態： 上皮樣
數量： 大量
ChaGo-K-1細胞Designations: ChaGo-K-1
Depositors: S Lippman, A Rabson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Tissue: bronchus
Disease: bronchogenic carcinoma
Cellular Products: hCG; human chorionic gonadotropin (alpha subunit only); estradiol; progesterone; mucin (apomucin, MUC-1, MUC-2)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. ChaGo-K-1細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 8,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number is 52, occurring at 40% with polyploidy at 3.8%., Over 2/3 of the chromosome complement consist of those with structural rearrangements (or markers) including 1q, t(1p,16q), del(3)(p13), t(5;14)(q35;q11), t(9q;?)1, t(9q;?)2, 12p+, 20p+, t(Xq,7p)., Twenty-one other markers are common to most cells. The normal chromosomes 1,3,5,9,14,15 and X are absent. All three E group chromosomes are paired., Y chromosome not detected by Q staining.
ChaGo-K-1細胞Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
LDH, 5
Me-2, 0
PGM1, 1
PGM3, 1
Age: 45 years
Gender: male
Comments: The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: ChaGo-K-1細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1179: Lieblich JM, et al. Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: Comparison of clonal strains from carcinomas of lung and placenta. J. Natl. Cancer Inst. 56: 911-917, 1976. PubMed: 62839
22525: Rabson AS, et al. Production of human chorionic gonadotropin in vitro by a cell line derived from a carcinoma of the lung. J. Natl. Cancer Inst. 50: 669-674, 1973. PubMed: 4708151
23102: Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822
23253: Tashjian AH Jr., et al. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc. Natl. Acad. Sci. USA 70: 1419-1422, 1973. PubMed: 4514312
