HMCB細胞
運輸方式： 凍存運輸
ATCC Number： CRL-9607?
器官來源： 皮膚
相關**： 黑色素瘤
細胞形態： 上皮樣
細胞類型： 上皮細胞
是否是腫瘤細胞： 1
物種來源： 人
生長狀態： 貼壁生長
數量： 大量
Designations: HMCB [ Human Melanoma Cell Bowes]
Depositors: Genentech, Inc.
HMCB細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: skin
Disease: melanoma
Cell Type: epithelial
Cellular Products: human tissue plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Comments: HMCB細胞The cells originally tested positive for mycoplasma.
After treatment with ciprofloxacin, the cells have remained negative for mycoplasma for 60 days.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with non-essential amino acids, 10 mM HEPES and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
HMCB細胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: culture medium 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:HMCB細胞ATCC 4-X
