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SK-MEL-1細胞

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產品名稱: SK-MEL-1細胞
產品型號: SK-MEL-1
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

SK-MEL-1細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉漿菌。主要的污染原因為無菌操作技術不當、操作室環境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環境、與品質良好之細胞來源和培養基配制是減低污染之*好方法。SK-MEL-1細胞何時須更換培養基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養基即可。


SK-MEL-1細胞  的詳細介紹

SK-MEL-1細胞

是否是腫瘤細胞: 1

物種來源: 人

器官來源: 皮膚

生長狀態: 懸浮生長

ATCC Number: HTB-67?

運輸方式: 凍存運輸

相關**: 惡性黑色素瘤

細胞形態: 其他

年限: 29 years

數量: 大量

Designations: SK-MEL-1

SK-MEL-1細胞Depositors: G Trempe, LJ Old

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: spherical


Source: Organ: skin

Disease: malignant melanoma

Derived from metastatic site: lymphatic system

Cellular Products: melanin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: SK-MEL-1細胞The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Applications: Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.

Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.

Tumorigenic: Yes

Antigen Expression: Blood Type A; Rh+

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12,13

D13S317: 11

D16S539: 11,12

D5S818: 12,13

D7S820: 12

THO1: 6

TPOX: 11

vWA: 16,17

Isoenzymes: AK-1, 1

SK-MEL-1細胞ES-D, 1

G6PD, B

GLO-I, 1-2

PGM1, 1

PGM3, 1

Age: 29 years

Gender: male

Ethnicity: Caucasian

Comments: F. Oettgen and associates isolated this line using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma.

Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.

Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: 2 to 3 times per week

Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 10 exp5 cells/ml and maintain at 2 X 10 exp5.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 1117: Oettgen HF, et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J. Natl. Cancer Inst. 41: 827-843, 1968. SK-MEL-1細胞PubMed: 4879578

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22816: Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447

23256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

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