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Hs 688(A).T細胞

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產品名稱: Hs 688(A).T細胞
產品型號: Hs 688(A).T
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

Hs 688(A).T細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉漿菌。主要的污染原因為無菌操作技術不當、操作室環境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環境、與品質良好之細胞來源和培養基配制是減低污染之*好方法。Hs 688(A).T細胞何時須更換培養基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養基即可。


Hs 688(A).T細胞  的詳細介紹

Hs 688(A).T細胞

運輸方式: 凍存運輸

器官來源: 皮膚

ATCC Number: CRL-7425?

相關**: 黑色素瘤

細胞形態: 成纖維樣

數量: 大量

是否是腫瘤細胞: 1

物種來源: 人

年限: 69 years

生長狀態: 貼壁生長

Designations: Hs 688(A).T

Hs 688(A).T細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: skin

Disease: melanoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: modal number = 46; range = 45 to 47

Age: 69 years

Gender: male

Ethnicity: Caucasian

Comments: Metastasis to inguinal lymph node also was cultured (see ATCC CRL-7426).

Hs 688(A).T細胞Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: Hs 688(A).T細胞A subcultivation ratio of 1:2 to 1:3 is recommended

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

derived from same individual:Hs 688(A).T細胞ATCC CRL-7426

滬公網安備 31011702004356號

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