GCT細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
年限： 29 years
ATCC Number： TIB-223?
相關(guān)**： 其他**
生長狀態(tài)： 貼壁生長
數(shù)量： 大量
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: GCT [Giant Cell Tumor]
Depositors: J Brennan
GCT細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Disease: fibrous histiocytoma
Derived from metastatic site: lung
Cellular Products: colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 9
GCT細(xì)胞D5S818: 13,15
D7S820: 11,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,18
Age: 29 years
Gender: male
Comments: The line produces CSA for human granulocyte precursors and EEA for erythroid precursor.
Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: GCT細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, add fresh medium, aspirate and dispense into new flasks.
References: 1181: Di Persio JF, et al. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species. Blood 51: 507-519, 1978. PubMed: 304748
1182: DiPersio JF, et al. The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. Blood 56: 717-727, 1980. PubMed: 6968234
1183: Abboud CN, et al. Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. GCT細(xì)胞Blood 58: 1148-1154, 1981. PubMed: 6975643
3550: Lichtman MA, Brennan JK. Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same. US Patent 4,135,975 dated Jan 23 1979
58064: Abboud CN, et al. Erythropoietic enhancing activity (EEA) secreted by the human cell line, GCT. J. Supramol. Struct. 13: 199-209, 1980. PubMed: 6972469
