GA-10 (Clone 20)細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： CRL-2394?

相關(guān)**： **瘤

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

年限： 41 years

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Designations: GA-10 (Clone 20)

GA-10 (Clone 20)細(xì)胞Depositors: AP Rapoport

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens deposited as human

Morphology: lymphoblast

Source: Disease: Burkitt's lymphoma

Cell Type: B lymphocyte;

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Antigen Expression: CD77 + (80-90%)

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,10

THO1: 9,9.3

GA-10 (Clone 20)細(xì)胞TPOX: 8,11

vWA: 16,20

Age: 41 years

Gender: male

Ethnicity: Caucasian, White

Comments: GA-10 (Clone 20) is a subclone of the GA-10 (ATCC CRL-2392) cell line derived by culturing the parental line on semi-solid medium conditioned by a feeder layer.

GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.

The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).

This clone of cells also expresses high levels of CD77 (80-90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).

GA-10 (Clone-20) cells exhibit marked sensitivity to Shiga Toxin (Stx) in cytotoxicity studies.

Propagation: GA-10 (Clone 20)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.

Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derived from same cell line:ATCC CRL-2392

derived from same cell line:GA-10 (Clone 20)細(xì)胞ATCC CRL-2393