143B PML BK TK細胞
年限： 13 year old
運輸方式： 凍存運輸
器官來源： 骨
ATCC Number： CRL-8304?
相關**： 骨肉瘤
是否是腫瘤細胞： 1
物種來源： 人
細胞形態： 成纖維樣
數量： 大量
生長狀態： 貼壁生長
143B PML BK TK細胞Designations: 143B PML BK TK
Depositors: Wistar Institute
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: bone
Disease: osteosarcoma
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DNA Profile (STR): 143B PML BK TK細胞Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Age: 13 year old
Gender: female
Ethnicity: Caucasian
Comments: Contains a thymidine kinase (TK+) plasmid (PMBKTK).
143B PML BK TK細胞Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: plasmid in bacteria:ATCC 39369
parental cell line:143B PML BK TK細胞ATCC CRL-8303
