G-361細(xì)胞

數(shù)量： 大量

器官來(lái)源： 皮膚

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

ATCC Number： CRL-1424?

相關(guān)**： 惡性黑色素瘤

細(xì)胞形態(tài)： 上皮樣

年限： 31 years *****

運(yùn)輸方式： 凍存運(yùn)輸

Designations: G-361

Depositors: PT Peebles

G-361細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: skin

Disease: malignant melanoma

Cellular Products: melanin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 11

G-361細(xì)胞D16S539: 15

D5S818: 11

D7S820: 8,11

THO1: 7,9

TPOX: 8,10

vWA: 15,17

Isoenzymes: G6PD, B

Age: 31 years *****

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. G-361細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week

References: 22209: . . Pediatr. G-361細(xì)胞Res. 12: 485, 1978.