MC-IXC細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-2270?
相關(guān)**： 神經(jīng)母細(xì)胞瘤
細(xì)胞形態(tài)： 成纖維樣
數(shù)量： 大量
器官來(lái)源： 大腦
年限： 14 years
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
MC-IXC細(xì)胞Designations: MC-IXC
Depositors: JL Biedler
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: brain
Disease: neuroblastoma
Derived from metastatic site: supra-orbital area
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: NOTE:MC-IXC細(xì)胞 MC-IXC was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. MC-IXC is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)MC-IXC or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of MC-IXC including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Antigen Expression: Blood Type O; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8
THO1: 9.3
TPOX: 9,11
vWA: 17,18
Cytogenetic Analysis: doubles minutes (DM) are prevalent
MC-IXC細(xì)胞Age: 14 years
Gender: female
Ethnicity: Caucasian
Comments: MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass.
The choline acetyltransferase activity of MC-IXC was approximately four times higher than the parental line but MC-IXC was negative for dopamine beta hydroxylase activity as was the parental line.
MC-IXC cells have a reported saturation density greater than 1 X 10 exp6 cells/sq cm.
The cells are fibroblast-like and grow to form a dense monolayer.
Floating cells are nonviable.
Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. MC-IXC細(xì)胞Incubate cultures at 37?C.
Subcultivation Ratio: 1:10 to 1:20
Medium Renewal: 2 to 3 times per week
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 36 hrs
References: 23018: Biedler JL, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 33: 2643-2652, 1973. PubMed: 4748425
23032: Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704
