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Hs 606細(xì)胞

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產(chǎn)品名稱: Hs 606細(xì)胞
產(chǎn)品型號(hào): Hs 606
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

Hs 606細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來(lái)源和培養(yǎng)基配制是減低污染之*好方法。Hs 606細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


Hs 606細(xì)胞  的詳細(xì)介紹

Hs 606細(xì)胞

數(shù)量: 大量

運(yùn)輸方式: 凍存運(yùn)輸

是否是腫瘤細(xì)胞: 1

物種來(lái)源: 人

器官來(lái)源: **

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

年限: 44 years

ATCC Number: CRL-7368?

相關(guān)**: 腫瘤

細(xì)胞形態(tài): 成纖維樣

Designations: Hs 606.T

Hs 606細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: mammary gland; breast

Disease: carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9,12

Hs 606細(xì)胞D16S539: 11,12

D5S818: 11,13

D7S820: 8,10

THO1: 7,9

TPOX: 8

vWA: 14,19

Age: 44 years

Gender: female

Ethnicity: Caucasian

Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 606細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37.0°C .


Subcultivation ratio: Hs 606細(xì)胞A subcultivation ratio of 1:2 to 1:3 is recommended.

Medium renewal: Every 2 to 3 days

Preservation: Freeze medium: complete growth medium, 90%; DMSO, 10%

liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002

Recommended serum: ATCC 30-2020

0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101

Phosphate-buffered saline: ATCC 30-2200

Cell culture tested DMSO: ATCC 4-X

滬公網(wǎng)安備 31011702004356號(hào)

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