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Hs 57.T細胞

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產品名稱: Hs 57.T細胞
產品型號: Hs 57.T
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

Hs 57.T細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉漿菌。主要的污染原因為無菌操作技術不當、操作室環境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環境、與品質良好之細胞來源和培養基配制是減低污染之*好方法。Hs 57.T細胞何時須更換培養基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養基即可。


Hs 57.T細胞  的詳細介紹

Hs 57.T細胞

ATCC Number: CRL-7037?

運輸方式: 瓶裝運輸

相關**: 其他**

器官來源: 肺

是否是腫瘤細胞: 1

物種來源: 人

數量: 大量

生長狀態: 貼壁生長

年限: 22 years

細胞形態: 成纖維樣

Hs 57.T細胞Designations: Hs 57.T

Biosafety Level: 1

Shipped: flask

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: lung

Disease: sarcoma or lymphoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 57.T細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 22 years

Gender: male

Ethnicity: Black

Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Note: This item is distributed only within the 50 United States. It is not available for international distribution.

Propagation: Hs 57.T細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Hs 57.T細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

滬公網安備 31011702004356號

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